By Alton Meister
Advances in Enzymology and comparable components of Molecular Biology is a seminal sequence within the box of biochemistry, providing researchers entry to authoritative stories of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, supplying an unequalled view of the old improvement of enzymology. The sequence bargains researchers the most recent realizing of enzymes, their mechanisms, reactions and evolution, roles in complicated organic procedure, and their program in either the laboratory and undefined. each one quantity within the sequence beneficial properties contributions through major pioneers and investigators within the box from world wide. All articles are rigorously edited to make sure thoroughness, caliber, and clarity.
With its wide variety of themes and lengthy historic pedigree, Advances in Enzymology and comparable parts of Molecular Biology can be utilized not just by way of scholars and researchers in molecular biology, biochemistry, and enzymology, but additionally by means of any scientist drawn to the invention of an enzyme, its houses, and its functions.
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Additional resources for Advances in Enzymology and Related Areas of Molecular Biology, Volume 13
USSING the resistance to the solutes. ) we have: Ordinarily gb (friction water/membrane) is of the same order as Gw (friction water/water), because of the condition that the membrane is nearly semipermeable (compare 34). We may then approach the ideal case: Mw (in,/’Mw(out) = auo/awi 07,141 Conformity with this equation is to be expected particularly when the membrane is a lipoid film where the water does not form a continuous phase, but penetrates as a dilute gas. If, however, the membrane structure is such that Gw >> gk (see equation V,13), flux ratios much larger than the ratio between the activities may result, and an uncritical use of equation (V,14) may lead to the erroneous assumption that we are dealing with active transport of water.
Consider for example the following case: The membrane is assumed to consist of elements or organelles shaped as small osmometers. These “osmometers” are closed by a thin semipermeable membrane toward the inside solution. The narrow stems of the osmometers open freely to the outside solution which is in this case pure solvent. The latter will pass through the stems to replenish the amount. lost through the semipermeable membranes. It is obvious that, a t a sufficiently high osmotic pressure, the linear rate of flow through the stems may become high enough to practically equal the diffusion rate in the direction opposite t o the flow.
Total molar concentration, irreapective of isotope. Unit usually arbitrary. If two tracers for the same substance are used simultaneously one is denoted by a n asterisk (*), the other by a dagger (I). Mole fraction of labeled molecules. Usually the specific activity in one of the solutions is put equal t o one at time zero. Volume, usually in liters. Example 1 (16): Substance present in much larger amount in the outside than in the inside solution. Influx and outflux equal. If the activity is initially present in the inside solution, we have: kz = - ( l / t ) In ( c ~ / c ~ ~ .
Advances in Enzymology and Related Areas of Molecular Biology, Volume 13 by Alton Meister